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| Catalog Number | Size | Price |
|---|---|---|
| LS-G3717-50 | 50 µg | $442 |
Rat Adiponectin Protein (Recombinant DDK (Flag)) (aa16-244) - LS-G3717
Rat Adiponectin Protein (Recombinant DDK (Flag)) (aa16-244) - LS-G3717
Description:
Adiponectin Protein LS-G3717 is a Recombinant Rat Adiponectin produced in HEK 293 Cells aa 16-244 with DDK (Flag) tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
Adiponectin Protein LS-G3717 is a Recombinant Rat Adiponectin produced in HEK 293 Cells aa 16-244 with DDK (Flag) tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
Adiponectin
Synonyms
ADIPOQ | Adiponectin | ADPN | ACDC | ADIPQTL1 | APM-1 | APM1 | ACRP30 | Gelatin-binding protein 28 | GBP28 | Gelatin-binding protein
Species
Rat
Modifications
Unmodified
Conjugations
Unconjugated
Tag
DDK (Flag)
Region
aa 16-244
Predicted Molecular Weight
~30kDa (SDS-PAGE)
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 90% by SDS-PAGE
Bio-Activity
Induces the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.
Endotoxin
Less than 0.1 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS
Storage
Store at 4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About Adiponectin
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Functional Assay
Effect of Adiponectin (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells. C2C12 myocytes were treated with 25 ug/ml for 15 minutes after serum starvation for 24 hours. 100 ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody. 1: 25 ug/ml of Adiponectin (rat) (rec.). 2: 25 ug/ml of a FLAG-tagged control protein. 3: phosphate-buffered saline.
Functional Assay
Effect of Adiponectin (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells. C2C12 myocytes were treated with 25 ug/ml for 15 minutes after serum starvation for 24 hours. 100 ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody. 1: 25 ug/ml of Adiponectin (rat) (rec.). 2: 25 ug/ml of a FLAG-tagged control protein. 3: phosphate-buffered saline.
Functional Assay
Effect of Adiponectin (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells. C2C12 myocytes were treated with 25 ug/ml for 15 minutes after serum starvation for 24 hours. 100 ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody. 1: 25 ug/ml of Adiponectin (rat) (rec.). 2: 25 ug/ml of a FLAG-tagged control protein. 3: phosphate-buffered saline.
Functional Assay
Effect of Adiponectin (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells. C2C12 myocytes were treated with 25 ug/ml for 15 minutes after serum starvation for 24 hours. 100 ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody. 1: 25 ug/ml of Adiponectin (rat) (rec.). 2: 25 ug/ml of a FLAG-tagged control protein. 3: phosphate-buffered saline.
Popular Adiponectin Proteins
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
Date Requested: 12/22/2024
