Target
NOG / Noggin
Synonyms
NOG | Symphalangism 1 (proximal) | SYNS1 | SYM1 | Noggin
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
Recombinant Mouse Noggin is a 46.4 kD disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains. The predicted N-terminal amino acid is Met.
Purification
Greater than 95% by SDS-PAGE
Usage Summary
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. Due to solubility reasons the protein should be kept at low pH. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA.
Bio-Activity
Mouse Noggin is able to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC5 chondrogenic cells with an ED50 of 1.0 - 2.0 ng/ml, corresponding to a specific activity of 5.0 x 10^5 - 1.0 x 10^6 units/mg.
Endotoxin
Less than 0.1 ng/µg of protein.
Storage
Store unopened at -20°C to -70°C. Store diluted at -20°C to -80°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee