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| Catalog Number | Size | Price |
|---|---|---|
| LS-G49405-50 | 50 µg | $364 |
| LS-G49405-500 | 500 µg | $1,379 |
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Mouse CD40L Protein (Recombinant Human IgG1 Fc, N-terminus) (Gly115-Leu260) - LS-G49405
Mouse CD40L Protein (Recombinant Human IgG1 Fc, N-terminus) (Gly115-Leu260) - LS-G49405
Description:
CD40L Protein LS-G49405 is a Recombinant Mouse CD40L produced in HEK 293 Cells Gly115-Leu260 with Human IgG1 Fc, N-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
CD40L Protein LS-G49405 is a Recombinant Mouse CD40L produced in HEK 293 Cells Gly115-Leu260 with Human IgG1 Fc, N-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
CD40L
Synonyms
CD40LG | CD154 | CD154 antigen | CD40-L | CD40 ligand | CD40L | gp39 | HIGM1 | IGM | IMD3 | T-BAM | TNFSF5 | CD40 antigen ligand | CD54 | HCD40L | T-B cell-activating molecule | T-cell antigen Gp39 | TNF-related activation protein
Species
Mouse
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
Human IgG1 Fc, N-terminus
Region
Gly115-Leu260
Predicted Molecular Weight
The recombinant mouse CD40LG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 406 amino acids and has a calculated molecular mass of 44.4 kDa. The apparent molecular mass of the monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 80 % as determined by SDS-PAGE
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized mouse CD40L-Fc at 10 µg/mL (100 µl/well) can bind biotinylated mouse CD40-Fch, The EC50 of biotinylated mouse CD40-Fch is 33-49 ng/mL.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD40L
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Binding
Measured by its binding ability in a functional ELISA. Immobilized mouse CD40L-Fc at 10 µg/mL (100 µl/well) can bind biotinylated mouse CD40-Fch, The EC50 of biotinylated mouse CD40-Fch is 33-49 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized mouse CD40L-Fc at 10 µg/mL (100 µl/well) can bind biotinylated mouse CD40-Fch, The EC50 of biotinylated mouse CD40-Fch is 33-49 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized mouse CD40L-Fc at 10 µg/mL (100 µl/well) can bind biotinylated mouse CD40-Fch, The EC50 of biotinylated mouse CD40-Fch is 33-49 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized mouse CD40L-Fc at 10 µg/mL (100 µl/well) can bind biotinylated mouse CD40-Fch, The EC50 of biotinylated mouse CD40-Fch is 33-49 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular CD40L Proteins
Source:
E. coli
Tag:
His, N-Terminal
Source:
E. coli
Tag:
6His, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/28/2024
Date Requested: 11/28/2024
