Target
CCL8 / MCP2
Synonyms
CCL8 | C-C motif chemokine 8 | HC14 | MCP2 | Monocyte chemotactic protein 2 | Scya10 | SCYA8 | Small-inducible cytokine A8 | MCP-2 | Chemokine (C-C motif) ligand 8
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 74 amino acid recombinant protein has a predicted molecular mass of approximately 8.48 kD. The DTT-reduced protein migrates at approximately 11 kDa and non-reduced protein migrates at approximately 12 kD by SDS-PAGE. The N-terminal amino acid is Gly.
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
Recombinant mouse CCL8 (MCP-2) chemoattracts Baf3-mCCR5 transfectants in a dose-dependent manner. The ED50 for this effect 50 is 0.2 - 1.2 µg/mL.
Endotoxin
Less than 0.01 ng/µg (determined by LAL method).
Presentation
0.22 µm filtered protein solution in PBS
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee