Target
VCAM1 / CD106
Synonyms
VCAM1 | CD106 | CD106 antigen | INCAM-100 | V-CAM 1 | VCAM-1
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 914 amino acid recombinant protein with a C-terminal human IgG1 Fc tag has a predicted molecular mass of approximately 101.4 kD. The protein migrates at approximately 130 kD by SDS-PAGE in DTT-reducing conditions and approximately 260 kD in non-reduci
Expression System
HEK 293 Cells
Purification
Greater than 95% by SDS-PAGE
Usage Summary
Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA.
Bio-Activity
U937 cells are able to bind to immobilized human VCAM-1-Fc chimera in a dose dependent manner. The ED50 = 0.5 - 2.5 µg/mL. Over 80% of the cells were able to bind human VCAM-1 at a dose greater than 5 µg/mL (100 µL/well coated).
Endotoxin
Less than 0.01 ng/µg (determined by LAL method).
Presentation
0.22 µm filtered protein solution in PBS
Storage
Store unopened at 2°C to 8°C for up to 3 months, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at 2°C to 8°C for up to 1 month, or at -20°to -70°C for up to 3 months. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee