Recombinant Protein

TNFRSF14 / CD270 / HVEM

Human

Unmodified

High Five Cells

Trichoplusia ni

Greater than 98% by SDS-PAGE and HPLC

Determined by its ability to neutralize 0.25 ng/ml of hTNFbeta induced cytotoxicity on murine L929 cells. The expected ED50 for this effect is 1.3-1.9 ug/ml of HVEM-Fc. Soluble HVEM, which tends to form disulfide-linked heterodimeric complexes in solution, can act as a “receptor decoy” resulting in inhibition of the activity of the HVEM ligands, LIGHT and TNF-ß.

Less than 0.1 ng/µg of protein (less than 1EU/µg).

Sterile filtered, lyophilized from 1X PBS, pH 7.2

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: Q92956 NCBI: NM_003820 NP_003811.2