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| Catalog Number | Size | Price |
|---|---|---|
| LS-G47212-10 | 10 µg | $255 |
| LS-G47212-100 | 100 µg | $364 |
| LS-G47212-1 | 1 mg | $1,452 |
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Human TNF Alpha Protein (Recombinant) (Val77-Leu233) - LS-G47212
Human TNF Alpha Protein (Recombinant) (Val77-Leu233) - LS-G47212
Description:
TNF Alpha Protein LS-G47212 is a Recombinant Human TNF Alpha produced in E. coli Val77-Leu233. It is biologically active. For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
TNF Alpha Protein LS-G47212 is a Recombinant Human TNF Alpha produced in E. coli Val77-Leu233. It is biologically active. For Research Use Only
Specifications
Type
Recombinant Protein
Target
TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Region
Val77-Leu233
Predicted Molecular Weight
The recombinant human TNF-a consists of 158 amino acids and has a predicted molecular mass of 17.4 kDa which is also estimated by SDS-PAGE.
Expression System
E. coli
Source Species
E. coli
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 5-50 pg/mL.
Endotoxin
Not Tested
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TNF Alpha
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Binding
Labeled biotin to TNF-alpha Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TNF-alpha Protein, Human, Recombinant, Biotinylated(Cat. 10602-HNAE-B) bound to Humira was 0.1 nM.
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Labeled biotin to TNF-alpha Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TNF-alpha Protein, Human, Recombinant, Biotinylated(Cat. 10602-HNAE-B) bound to Humira was 0.1 nM.
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Labeled biotin to TNF-alpha Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TNF-alpha Protein, Human, Recombinant, Biotinylated(Cat. 10602-HNAE-B) bound to Humira was 0.1 nM.
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Labeled biotin to TNF-alpha Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TNF-alpha Protein, Human, Recombinant, Biotinylated(Cat. 10602-HNAE-B) bound to Humira was 0.1 nM.
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular TNF Alpha Proteins
Source:
Wheat
Tag:
GST, N-terminus
Source:
Human
Tag:
His, N-Terminal
Source:
E. coli
Tag:
His, N-Terminal
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/25/2024
Date Requested: 12/25/2024
