Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Location
Corporate Headquarters
Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States
Telephone Numbers
Customer Service: (800) 227-6666 / (650) 697-3600
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-G137542-10 | 10 µg (0.2 mg/ml) | $306 |
| LS-G137542-50 | 50 µg (0.2 mg/ml) | $363 |
| LS-G137542-100 | 100 µg | $485 |
| LS-G137542-500 | 500 µg | $1,000 |
Human TNF Alpha Protein (Recombinant) - LS-G137542
Human TNF Alpha Protein (Recombinant) - LS-G137542
Description:
TNF Alpha Protein LS-G137542 is a Recombinant Human TNF Alpha. It is biologically active and is low in endotoxin; Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
TNF Alpha Protein LS-G137542 is a Recombinant Human TNF Alpha. It is biologically active and is low in endotoxin; Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Species
Human
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 157 amino acid recombinant protein has a predicted molecular mass of 17,352 Da. The DTT-reduced protein and non-reduced protein migrate at approximately 16kDa by SDS-PAGE. The N-terminal amino acid is Val.
Source Species
E. coli
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
The ED50 is equal to0.02 €“ 0.1 ng/mL as determined by the dose-dependent cytotoxicity of L929 cells stimulated with actinomycin D. The specific activity of recombinant human TNF-α is approximately 5.35 x 10^4 IU/µg when compared against the 3rd WHO International Standard for Human TNF-α (NIBSC code: 12/154).
Endotoxin
Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method).
Presentation
0.22 µm filtered protein solution, pH 7.2, 10 mM NaH2PO4, 150 mM NaCl
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TNF Alpha
Publications (0)
Customer Reviews (0)
Images
Cytotoxic Assay
Human TNF-α cytotoxicity on L929 cells.
Cytotoxic Assay
Human TNF-α cytotoxicity on L929 cells.
Cytotoxic Assay
Human TNF-α cytotoxicity on L929 cells.
Cytotoxic Assay
Human TNF-α cytotoxicity on L929 cells.
Popular TNF Alpha Proteins
Source:
Wheat
Tag:
GST, N-terminus
Source:
Human
Tag:
His, N-Terminal
Source:
E. coli
Tag:
His, N-Terminal
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
Date Requested: 12/21/2024
