Target
SERPING1 / C1 Inhibitor
Synonyms
SERPING1 | C1-inhibiting factor | C1IN | C1 Inh | C1-INH | C1INH | C1NH | C1 Inhibitor | HAE1 | HAE2 | Plasma protease C1 inhibitor | C1 esterase inhibitor | Serpin G1
Conjugations
Unconjugated
Applications
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The secreted recombinant human SERPING1 consists of 489 amino acids and predictes a molecular mass of 54.3 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 110 kDa due to glycosylation.
Expression System
HEK 293 Cells
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its ability to inhibit recombinant human complement component C1s cleavage of a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-K-SBzl). The IC50 is < 15 nM.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About SERPING1 / C1 Inhibitor