Target
PPP1CA / PP1-Alpha
Synonyms
PPP1CA | PP-1A | pp1C alpha | PP1 alpha | PP1-Alpha | PP1alpha | PPP1A | Protein phosphatase 1 alpha
Conjugations
Unconjugated
AA Sequence
MGSSHHHHHHSSGLVPRGSHMSDSEKLNLDSIIGRLLEVQGSRPGKNVQLTENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKNKGKYGQFSGLNPGGRPITPPRNSAKAKK
Expression System
E. coli
Purification
Greater than 80% by SDS-PAGE
Bio-Activity
Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10 mM of substrate.
Presentation
50 mM Tris-HCl, pH 8.5, 0.2 M NaCl, 1 mM DTT, 0.1 mM PMSF, 1 mM MnCl2, 50% Glycerol
Storage
Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee