Target
Osteonectin / SPARC
Synonyms
SPARC | Basement-membrane protein 40 | BM-40 | Cysteine-rich protein | ON | Osteonectin
Conjugations
Unconjugated
Predicted Molecular Weight
36.1 kDa
AA Sequence
MSYYHHHHHHDYDIPTTENLYFQGAMGSAPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI
Purification
Greater than 95% by SDS-PAGE and HPLC
Usage Summary
A single non-glycosylated polypeptide chain containing 314 amino acids, with expression vector sequence (containing 6 × His tag).
Bio-Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 µg/mL, corresponding to a specific activity of > 333 IU/mg.
Endotoxin
Less than 1 EU/µg (determined by LAL method).
Presentation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4
Reconstitution
Briefly centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
Storage
Store lyophilized at 2°C to 8°C for short term storage. Store lyophilized at -20°C for long term storage. Store reconstituted at 2°C to 8°C for up to 1 week. Aliquot and store reconstituted at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About Osteonectin / SPARC