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Catalog Number Size Price
LS-G3911-10 10 µg $414 
NTN1 / Netrin 1 Protein - Netrin-1 (human):Fc (human) (rec.) triggers a DCC-dependent phosphorylation of ERK1/2. Method: HEK 293 cells (Control) or HEK 293 expressing the netrin-1 receptor DCC were incubated with netrin-1 (human):Fc (human) (rec.) (5 nM) for the indicated time points (shown in minutes). Antibodies against DCC, total ERK1/2 or phospho-ERK1/2 were used and visualized with a chemiluminescence detection system. Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon
NTN1 / Netrin 1 Protein - Netrin-1 (human):Fc (human) (rec.) induces outgrowth of the commisural axon.Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250ng/ml). Axons were then stained with an anti-beta-tubulin antibody. Picture courtesy of Dr. Veronique Corset, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon
NTN1 / Netrin 1 Protein - Netrin-1 (human):Fc (human) (rec.) triggers a DCC-dependent phosphorylation of ERK1/2. Method: HEK 293 cells (Control) or HEK 293 expressing the netrin-1 receptor DCC were incubated with netrin-1 (human):Fc (human) (rec.) (5 nM) for the indicated time points (shown in minutes). Antibodies against DCC, total ERK1/2 or phospho-ERK1/2 were used and visualized with a chemiluminescence detection system. Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon
NTN1 / Netrin 1 Protein - Netrin-1 (human):Fc (human) (rec.) induces outgrowth of the commisural axon.Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250ng/ml). Axons were then stained with an anti-beta-tubulin antibody. Picture courtesy of Dr. Veronique Corset, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon
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Human NTN1 / Netrin 1 Protein (Recombinant Human IgG1 Fc) (aa25-604) - LS-G3911

Human NTN1 / Netrin 1 Protein (Recombinant Human IgG1 Fc) (aa25-604) - LS-G3911

Description:
NTN1 / Netrin 1 Protein LS-G3911 is a Recombinant Human NTN1 / Netrin 1 produced in HEK 293 Cells aa 25-604 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only
Price
Catalog Number
$414
LS-G3911-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Description:
NTN1 / Netrin 1 Protein LS-G3911 is a Recombinant Human NTN1 / Netrin 1 produced in HEK 293 Cells aa 25-604 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only

Specifications

Type
Recombinant Protein
Target
NTN1 / Netrin 1
Synonyms
NTN1 | Netrin 1 | Netrin 1, mouse, homolog of | Netrin-1 | NTN1L
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Human IgG1 Fc
Region
aa 25-604
Predicted Molecular Weight
~90kDa (SDS-PAGE)
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Induces axon outgrowth.
Endotoxin
Less than 0.1 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS, Trehalose, Brij-35
Reconstitution
Reconstitute with 100 µl sterile distilled water.
Storage
Store lyophilized at -20°C for up to 6 months. Once reconstituted store at 4°C for immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About NTN1 / Netrin 1
O95631 NM_004822 NP_004813.2

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Images

Functional Assay

NTN1 / Netrin 1 Protein - Netrin-1 (human):Fc (human) (rec.) triggers a DCC-dependent phosphorylation of ERK1/2. Method: HEK 293 cells (Control) or HEK 293 expressing the netrin-1 receptor DCC were incubated with netrin-1 (human):Fc (human) (rec.) (5 nM) for the indicated time points (shown in minutes). Antibodies against DCC, total ERK1/2 or phospho-ERK1/2 were used and visualized with a chemiluminescence detection system. Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon
Netrin-1 (human):Fc (human) (rec.) triggers a DCC-dependent phosphorylation of ERK1/2. Method: HEK 293 cells (Control) or HEK 293 expressing the netrin-1 receptor DCC were incubated with netrin-1 (human):Fc (human) (rec.) (5 nM) for the indicated time points (shown in minutes). Antibodies against DCC, total ERK1/2 or phospho-ERK1/2 were used and visualized with a chemiluminescence detection system. Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon

Functional Assay

NTN1 / Netrin 1 Protein - Netrin-1 (human):Fc (human) (rec.) induces outgrowth of the commisural axon.Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250ng/ml). Axons were then stained with an anti-beta-tubulin antibody. Picture courtesy of Dr. Veronique Corset, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon
Netrin-1 (human):Fc (human) (rec.) induces outgrowth of the commisural axon.Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250ng/ml). Axons were then stained with an anti-beta-tubulin antibody. Picture courtesy of Dr. Veronique Corset, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon

Functional Assay

NTN1 / Netrin 1 Protein - Netrin-1 (human):Fc (human) (rec.) triggers a DCC-dependent phosphorylation of ERK1/2. Method: HEK 293 cells (Control) or HEK 293 expressing the netrin-1 receptor DCC were incubated with netrin-1 (human):Fc (human) (rec.) (5 nM) for the indicated time points (shown in minutes). Antibodies against DCC, total ERK1/2 or phospho-ERK1/2 were used and visualized with a chemiluminescence detection system. Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon
Netrin-1 (human):Fc (human) (rec.) triggers a DCC-dependent phosphorylation of ERK1/2. Method: HEK 293 cells (Control) or HEK 293 expressing the netrin-1 receptor DCC were incubated with netrin-1 (human):Fc (human) (rec.) (5 nM) for the indicated time points (shown in minutes). Antibodies against DCC, total ERK1/2 or phospho-ERK1/2 were used and visualized with a chemiluminescence detection system. Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon

Functional Assay

NTN1 / Netrin 1 Protein - Netrin-1 (human):Fc (human) (rec.) induces outgrowth of the commisural axon.Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250ng/ml). Axons were then stained with an anti-beta-tubulin antibody. Picture courtesy of Dr. Veronique Corset, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon
Netrin-1 (human):Fc (human) (rec.) induces outgrowth of the commisural axon.Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250ng/ml). Axons were then stained with an anti-beta-tubulin antibody. Picture courtesy of Dr. Veronique Corset, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon

Functional Assay

NTN1 / Netrin 1 Protein - Netrin-1 (human):Fc (human) (rec.) triggers a DCC-dependent phosphorylation of ERK1/2. Method: HEK 293 cells (Control) or HEK 293 expressing the netrin-1 receptor DCC were incubated with netrin-1 (human):Fc (human) (rec.) (5 nM) for the indicated time points (shown in minutes). Antibodies against DCC, total ERK1/2 or phospho-ERK1/2 were used and visualized with a chemiluminescence detection system. Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon
Netrin-1 (human):Fc (human) (rec.) triggers a DCC-dependent phosphorylation of ERK1/2. Method: HEK 293 cells (Control) or HEK 293 expressing the netrin-1 receptor DCC were incubated with netrin-1 (human):Fc (human) (rec.) (5 nM) for the indicated time points (shown in minutes). Antibodies against DCC, total ERK1/2 or phospho-ERK1/2 were used and visualized with a chemiluminescence detection system. Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon

Functional Assay

NTN1 / Netrin 1 Protein - Netrin-1 (human):Fc (human) (rec.) induces outgrowth of the commisural axon.Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250ng/ml). Axons were then stained with an anti-beta-tubulin antibody. Picture courtesy of Dr. Veronique Corset, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon
Netrin-1 (human):Fc (human) (rec.) induces outgrowth of the commisural axon.Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250ng/ml). Axons were then stained with an anti-beta-tubulin antibody. Picture courtesy of Dr. Veronique Corset, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon

Functional Assay

NTN1 / Netrin 1 Protein - Netrin-1 (human):Fc (human) (rec.) triggers a DCC-dependent phosphorylation of ERK1/2. Method: HEK 293 cells (Control) or HEK 293 expressing the netrin-1 receptor DCC were incubated with netrin-1 (human):Fc (human) (rec.) (5 nM) for the indicated time points (shown in minutes). Antibodies against DCC, total ERK1/2 or phospho-ERK1/2 were used and visualized with a chemiluminescence detection system. Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon
Netrin-1 (human):Fc (human) (rec.) triggers a DCC-dependent phosphorylation of ERK1/2. Method: HEK 293 cells (Control) or HEK 293 expressing the netrin-1 receptor DCC were incubated with netrin-1 (human):Fc (human) (rec.) (5 nM) for the indicated time points (shown in minutes). Antibodies against DCC, total ERK1/2 or phospho-ERK1/2 were used and visualized with a chemiluminescence detection system. Picture courtesy of Nicolas Rama, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon

Functional Assay

NTN1 / Netrin 1 Protein - Netrin-1 (human):Fc (human) (rec.) induces outgrowth of the commisural axon.Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250ng/ml). Axons were then stained with an anti-beta-tubulin antibody. Picture courtesy of Dr. Veronique Corset, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon
Netrin-1 (human):Fc (human) (rec.) induces outgrowth of the commisural axon.Method: Dorsal spinal cords were dissected out from E13 rat embryos and cultured in collagen matrix in the presence or absence of netrin-1 (250ng/ml). Axons were then stained with an anti-beta-tubulin antibody. Picture courtesy of Dr. Veronique Corset, Prof. Patrick Mehlen lab, Centre Leon Berard, Lyon

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Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/26/2024