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| Catalog Number | Size | Price |
|---|---|---|
| LS-G47818-100 | 100 µg | $480 |
| LS-G47818-1 | 1 mg | $2,324 |
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Human KDR / VEGFR2 / FLK1 Protein (Recombinant His,C-terminus) (Met1-Glu764) - LS-G47818
Human KDR / VEGFR2 / FLK1 Protein (Recombinant His,C-terminus) (Met1-Glu764) - LS-G47818
Description:
KDR / VEGFR2 / FLK1 Protein LS-G47818 is a Recombinant Human KDR / VEGFR2 / FLK1 produced in HEK 293 Cells Met1-Glu764 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
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(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
KDR / VEGFR2 / FLK1 Protein LS-G47818 is a Recombinant Human KDR / VEGFR2 / FLK1 produced in HEK 293 Cells Met1-Glu764 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
KDR / VEGFR2 / FLK1
Synonyms
KDR | CD309 | Fetal liver kinase 1 | Fetal liver kinase-1 | FLK-1 | FLK1 | Kinase insert domain receptor | Quek1 | VEGFR | VEGFR2 | Soluble VEGFR2 | VEGF receptor 2 | VEGFR-2 | CD309 antigen | VEGF-R2
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His,C-terminus
Region
Met1-Glu764
Predicted Molecular Weight
The secreted recombinant human VEGFR2 consists of 756 amino acids and predicts a molecular mass of 84.6 kDa. The apparent molecular mass of rhVEGFR2 is approximatly 120-130 in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 97 % as determined by SDS-PAGE
Bio-Activity
Using the Octet RED System,the affinity constant (Kd) of human VEGFR2-his bound to biotinylated human VEGF165 was 5.9nM.2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 10-50µg/mL in the presence of 10 ng/mL recombinant human VEGF165.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About KDR / VEGFR2 / FLK1
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Binding
Using the Octet RED System, the affinity constant (Kd) of VEGFR2 / KDR Protein, Human, Recombinant (His Tag) bound to Anti-VEGFR2 Antibody was 1.9 µM.
Proliferation
Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED50 for this effect is typically 10-50µg/mL in the presence of 10 ng/mL recombinant human VEGF165.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Using the Octet RED System, the affinity constant (Kd) of VEGFR2 / KDR Protein, Human, Recombinant (His Tag) bound to Anti-VEGFR2 Antibody was 1.9 µM.
Proliferation
Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED50 for this effect is typically 10-50µg/mL in the presence of 10 ng/mL recombinant human VEGF165.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Using the Octet RED System, the affinity constant (Kd) of VEGFR2 / KDR Protein, Human, Recombinant (His Tag) bound to Anti-VEGFR2 Antibody was 1.9 µM.
Proliferation
Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED50 for this effect is typically 10-50µg/mL in the presence of 10 ng/mL recombinant human VEGF165.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Using the Octet RED System, the affinity constant (Kd) of VEGFR2 / KDR Protein, Human, Recombinant (His Tag) bound to Anti-VEGFR2 Antibody was 1.9 µM.
Proliferation
Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED50 for this effect is typically 10-50µg/mL in the presence of 10 ng/mL recombinant human VEGF165.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular KDR / VEGFR2 / FLK1 Proteins
Source:
Human
Tag:
Human IgG Fc, C-terminus
Source:
E. coli
Tag:
His-S
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
