Target
HGF / Hepatocyte Growth Factor
Synonyms
HGF | DFNB39 | Hepatocyte growth factor | Hepatopoeitin-A | Hepapoietin A | Hepatopoietin-A | Scatter factor) | SF | F-TCF | HGFB | HPTA | Scatter factor
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The alpha and beta chains contains 463 and 234 amino acids and have a predicted molecular mass of approximately 53.6 and 26 kD respectively. The predicted N-terminal amino acids are Gln and Val.
Expression System
Insect Cells
Purification
Greater than 98% by SDS-PAGE and HPLC
Usage Summary
Reconstitute in water to a concentration of 0.5 mg/ml. Do not vortex. Allow the reconstituted vial to sit at room temperature for 1 hour before use. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA.
Bio-Activity
ED50 is equal to20 - 40 ng/ml, corresponding to a specific activity of 2.5 - 5.0 x 10^4 units/mg, as determined by induction of 4MBr-5 cell proliferation.
Endotoxin
Less than 0.1 ng/µg of protein.
Storage
Store unopened at -20°C to -70°C. Store diluted at -20°C to -80°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About HGF / Hepatocyte Growth Factor