Recombinant Protein

FN1 / Fibronectin

Human

Unmodified

62.7 kDa

E. coli

E. coli

Greater than 95% by SDS-PAGE

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.; FUNCTION

Specific activity as determined by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells is greater than 50%. When 1×10^5 cells/well are added to plates coated with fibronectin (7-13 ng/mL and 100µL/well),approximately 50%-80% will adhere specifically after 30 minutes at 37°C.

Less than 1.0 EU/µg protein (determined by LAL method).

Lyophilized from 0.2 µm filtered 12.5 mM Sodium Citrate, pH 6.2, 1.25% Sucrose

Store at -80°C for up to 1 year. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: P02751 NCBI: NM_002026 NP_002017.1