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| Catalog Number | Size | Price |
|---|---|---|
| LS-G3967-10 | 10 µg | $435 |
Human FASLG / Fas Ligand Protein (Recombinant DDK (Flag)-Human ACRP30headless aa 18-111) (aa139-281) - LS-G3967
Human FASLG / Fas Ligand Protein (Recombinant DDK (Flag)-Human ACRP30headless aa 18-111) (aa139-281) - LS-G3967
Description:
FASLG / Fas Ligand Protein LS-G3967 is a Recombinant Human FASLG / Fas Ligand produced in HEK 293 Cells aa 139-281 with DDK (Flag)-Human ACRP30headless aa 18-111 tag(s). It is low in endotoxin; Less than 0.02 EU/µg protein (determined by LAL method). For Research Use Only
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Overview
Description:
FASLG / Fas Ligand Protein LS-G3967 is a Recombinant Human FASLG / Fas Ligand produced in HEK 293 Cells aa 139-281 with DDK (Flag)-Human ACRP30headless aa 18-111 tag(s). It is low in endotoxin; Less than 0.02 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
FASLG / Fas Ligand
Synonyms
FASLG | ALPS1B | Apoptosis antigen ligand | APTL | CD95L | CD95 ligand | CD178 antigen | CD95-L | Fas antigen ligand | Fas ligand | FASL | TNFSF6 | APT1LG1 | CD178
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
DDK (Flag)-Human ACRP30headless aa 18-111
Region
aa 139-281
Predicted Molecular Weight
~40kDa (SDS-PAGE)
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Induces apoptosis of human Jurkat T cells at a concentration of <0.2ng/ml.
Endotoxin
Less than 0.02 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute with 100 µl sterile water.
Storage
Store lyophilized at -20°C for up to 6 months. Once reconstituted store at 4°C for immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
About FASLG / Fas Ligand
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Functional Assay
Oligomerization of FasL (h) efficiently triggers Jurkat cell death.Method: Jurkat cells were treated O/N with the indicated concentrations of FasL (human) (multimeric) (rec.) (rec.) (rec.) or FasL, Soluble (human) (rec.) + TNF ligands enhancer (2 fold-dilutions, first concentration of 1000ng/ml). Cell death was quantified using PMS/MTS. The oligomeric FasL recombinant proteins (FasL (human) (multimeric) (rec.), Fc:FasL, Soluble (human) and FasL, Soluble (human) + TNF ligands enhancer) kill Jurkat cells at IC50 <0.2ng/ml.
Functional Assay
Oligomerization of FasL (h) efficiently triggers Jurkat cell death.Method: Jurkat cells were treated O/N with the indicated concentrations of FasL (human) (multimeric) (rec.) (rec.) (rec.) or FasL, Soluble (human) (rec.) + TNF ligands enhancer (2 fold-dilutions, first concentration of 1000ng/ml). Cell death was quantified using PMS/MTS. The oligomeric FasL recombinant proteins (FasL (human) (multimeric) (rec.), Fc:FasL, Soluble (human) and FasL, Soluble (human) + TNF ligands enhancer) kill Jurkat cells at IC50 <0.2ng/ml.
Functional Assay
Oligomerization of FasL (h) efficiently triggers Jurkat cell death.Method: Jurkat cells were treated O/N with the indicated concentrations of FasL (human) (multimeric) (rec.) (rec.) (rec.) or FasL, Soluble (human) (rec.) + TNF ligands enhancer (2 fold-dilutions, first concentration of 1000ng/ml). Cell death was quantified using PMS/MTS. The oligomeric FasL recombinant proteins (FasL (human) (multimeric) (rec.), Fc:FasL, Soluble (human) and FasL, Soluble (human) + TNF ligands enhancer) kill Jurkat cells at IC50 <0.2ng/ml.
Functional Assay
Oligomerization of FasL (h) efficiently triggers Jurkat cell death.Method: Jurkat cells were treated O/N with the indicated concentrations of FasL (human) (multimeric) (rec.) (rec.) (rec.) or FasL, Soluble (human) (rec.) + TNF ligands enhancer (2 fold-dilutions, first concentration of 1000ng/ml). Cell death was quantified using PMS/MTS. The oligomeric FasL recombinant proteins (FasL (human) (multimeric) (rec.), Fc:FasL, Soluble (human) and FasL, Soluble (human) + TNF ligands enhancer) kill Jurkat cells at IC50 <0.2ng/ml.
Popular FASLG / Fas Ligand Proteins
Source:
E. coli
Tag:
His, N-Terminal
Source:
Hamster-Chinese
Tag:
His, N-Terminal
Source:
Human
Tag:
DDK (Flag)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
