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| Catalog Number | Size | Price |
|---|---|---|
| LS-G47281-100 | 100 µg | $441 |
| LS-G47281-1 | 1 mg | $1,991 |
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Human ERBB2 / HER2 Protein (Recombinant Human IgG Fc, C-terminus) (Met1-Thr652) - LS-G47281
Human ERBB2 / HER2 Protein (Recombinant Human IgG Fc, C-terminus) (Met1-Thr652) - LS-G47281
Description:
ERBB2 / HER2 Protein LS-G47281 is a Recombinant Human ERBB2 / HER2 produced in HEK 293 Cells Met1-Thr652 with Human IgG Fc, C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
ERBB2 / HER2 Protein LS-G47281 is a Recombinant Human ERBB2 / HER2 produced in HEK 293 Cells Met1-Thr652 with Human IgG Fc, C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
ERBB2 / HER2
Synonyms
ERBB2 | C-erb B2/neu protein | CD340 | HER2 | Herstatin | MLN 19 | HER-2 | Neu | NGL | Proto-oncogene c-ErbB-2 | Proto-oncogene Neu | CD340 antigen | MLN19 | p185erbB2 | TKR1
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
Human IgG Fc, C-terminus
Region
Met1-Thr652
Predicted Molecular Weight
The recombinant human ErbB2/Fc chimera is a disulfide-linked homodimeric protein generated by proteolytic removal of the signal peptide. The monomer comprises 868 amino acids and has a calculated molecular mass of 96.1 kDa. As a result of glycosylation, the monomer migrates as an approximately 130-140 kDa protein in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 90 % as determined by SDS-PAGE
Bio-Activity
Measured by its binding ability in a functional ELISA.Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80
Reconstitution
Reconstitute in 400ul of sterile water to a concentration of 0.25mg/ml
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ERBB2 / HER2
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Binding
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
Proliferation
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
Proliferation
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
Proliferation
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
Proliferation
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular ERBB2 / HER2 Proteins
Source:
Human
Tag:
6His, N-terminus
Source:
E. coli
Tag:
His-GST
Source:
E. coli
Tag:
His, N-Terminal
Source:
Human
Tag:
Fc, C-terminus
Source:
Human
Tag:
His,C-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
