Recombinant Protein

ERBB2 / HER2

Human

Unmodified

The recombinant human ErbB2 comprises 641 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, rh ErbB2 migrates as an approximately 100-110 kDa protein in SDS-PAGE under reducing conditions.

HEK 293 Cells

Human

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.Immobilized human Erbb2 at 2.5 µg/ml (100 µl/well) can bind Herceptin with a linear ranger of 1.28-32 ng/ml. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.3-1.8 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.

Less than 1.0 EU/µg protein (determined by LAL method).

Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to included instructions for detailed information.

Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: P04626 NCBI: NM_004448 NP_004439.2