Target
CD40L
Synonyms
CD40LG | CD154 | CD154 antigen | CD40-L | CD40 ligand | CD40L | gp39 | HIGM1 | IGM | IMD3 | T-BAM | TNFSF5 | CD40 antigen ligand | CD54 | HCD40L | T-B cell-activating molecule | T-cell antigen Gp39 | TNF-related activation protein
Conjugations
Unconjugated
Predicted Molecular Weight
16.308 kDa
AA Sequence
First5N-terminalaminoacids:MQKGD
Purification
Greater than 95% by RP-HPLC, FPLC, SDS-PAGE
Bio-Activity
Recombinant human CD40 Ligand (CD40L/CD154/TRAP) is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5-10 ng/ml, corresponding to a Specific Activity of 1.0 x 105 IU/mg.
Endotoxin
Less than 1 EU/µg (determined by LAL method).
Presentation
Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0, 250 mM NaCl
Reconstitution
Reconstitute in sterile 18 MO-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Storage
Store lyophilized at room temperature for up to 3 weeks, or preferably at -18°C or below. Store reconstituted at 4°C for up to 1 week. Store at -18°C or below for long term storage. Add a carrier protein (0.1% HSA or BSA) for long term storage.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee