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| Catalog Number | Size | Price |
|---|---|---|
| LS-G48667-50 | 50 µg | $364 |
| LS-G48667-500 | 500 µg | $1,285 |
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Human CD40L Protein (Recombinant Human IgG1 Fc, N-terminus) (Met113-Leu261) - LS-G48667
Human CD40L Protein (Recombinant Human IgG1 Fc, N-terminus) (Met113-Leu261) - LS-G48667
Description:
CD40L Protein LS-G48667 is a Recombinant Human CD40L produced in HEK 293 Cells Met113-Leu261 with Human IgG1 Fc, N-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
CD40L Protein LS-G48667 is a Recombinant Human CD40L produced in HEK 293 Cells Met113-Leu261 with Human IgG1 Fc, N-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
CD40L
Synonyms
CD40LG | CD154 | CD154 antigen | CD40-L | CD40 ligand | CD40L | gp39 | HIGM1 | IGM | IMD3 | T-BAM | TNFSF5 | CD40 antigen ligand | CD54 | HCD40L | T-B cell-activating molecule | T-cell antigen Gp39 | TNF-related activation protein
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
Human IgG1 Fc, N-terminus
Region
Met113-Leu261
Predicted Molecular Weight
The recombinant human CD40L/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 386 amino acids and has a predicted molecular mass of 42.8 kDa. The apparent molecular mass of rh CD40L/Fc monomer is approximately 45 kDa in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 90 % as determined by SDS-PAGE
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized human CD40 at 2 µg/ml (100 µl/well) can bind human CD40L Fc Chimera with a linear ranger of 1.28-32 ng/ml.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD40L
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Binding
Measured by its binding ability in a functional ELISA. Immobilized human CD40 at 2 µg/ml (100 µl/well) can bind human CD40L Fc Chimera with a linear ranger of 1.28-32 ng/ml.
Proliferation
Measured in a cell proliferation assay using Ramos.2G6.4C10 Burkitt's lymphoma cells. The ED50 for this effect is typically 10-50 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human CD40 at 2 µg/ml (100 µl/well) can bind human CD40L Fc Chimera with a linear ranger of 1.28-32 ng/ml.
Proliferation
Measured in a cell proliferation assay using Ramos.2G6.4C10 Burkitt's lymphoma cells. The ED50 for this effect is typically 10-50 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human CD40 at 2 µg/ml (100 µl/well) can bind human CD40L Fc Chimera with a linear ranger of 1.28-32 ng/ml.
Proliferation
Measured in a cell proliferation assay using Ramos.2G6.4C10 Burkitt's lymphoma cells. The ED50 for this effect is typically 10-50 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human CD40 at 2 µg/ml (100 µl/well) can bind human CD40L Fc Chimera with a linear ranger of 1.28-32 ng/ml.
Proliferation
Measured in a cell proliferation assay using Ramos.2G6.4C10 Burkitt's lymphoma cells. The ED50 for this effect is typically 10-50 ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular CD40L Proteins
Source:
E. coli
Tag:
His, N-Terminal
Source:
E. coli
Tag:
6His, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/28/2024
Date Requested: 11/28/2024
