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| Catalog Number | Size | Price |
|---|---|---|
| LS-G48639-100 | 100 µg | $410 |
| LS-G48639-1 | 1 mg | $2,261 |
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Human CD40L Protein (Recombinant His,C-terminus) (Glu108-Leu261) - LS-G48639
Human CD40L Protein (Recombinant His,C-terminus) (Glu108-Leu261) - LS-G48639
Description:
CD40L Protein LS-G48639 is a Recombinant Human CD40L produced in E. coli Glu108-Leu261 with His,C-terminus tag(s). It is biologically active. For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
CD40L Protein LS-G48639 is a Recombinant Human CD40L produced in E. coli Glu108-Leu261 with His,C-terminus tag(s). It is biologically active. For Research Use Only
Specifications
Type
Recombinant Protein
Target
CD40L
Synonyms
CD40LG | CD154 | CD154 antigen | CD40-L | CD40 ligand | CD40L | gp39 | HIGM1 | IGM | IMD3 | T-BAM | TNFSF5 | CD40 antigen ligand | CD54 | HCD40L | T-B cell-activating molecule | T-cell antigen Gp39 | TNF-related activation protein
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His,C-terminus
Region
Glu108-Leu261
Predicted Molecular Weight
The recombinant human CD40L consists of 161 amino acids and has a calculated molecular mass of 17.7 kDa. It migrates as an 18 kDa band in SDS-PAGE under reducing conditions.
Expression System
E. coli
Source Species
E. coli
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized human CD40L at 10 µg/ml (100 µl/well) can bind human CD40 with a linear range of 15.6-500 ng/ml. Immobilized human CD40L at 10 µg/ml (100 µl/well) can bind human CD40 / Fc with a linear range of 7.8-125 ng/ml.
Endotoxin
Not Tested
Presentation
Lyophilized from sterile PBS, pH 7.4, 10% glycerol, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD40L
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Binding
Measured by its binding ability in a functional ELISA. Immobilized human CD40L at 10 µg/ml (100 µl/well) can bind human CD40 / Fc with a linear range of 7.8-125 ng/ml.
Binding
Measured by its binding ability in a functional ELISA. Immobilized human CD40L at 10 µg/ml (100 µl/well) can bind human CD40 with a linear range of 15.6-500 ng/ml.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human CD40L at 10 µg/ml (100 µl/well) can bind human CD40 / Fc with a linear range of 7.8-125 ng/ml.
Binding
Measured by its binding ability in a functional ELISA. Immobilized human CD40L at 10 µg/ml (100 µl/well) can bind human CD40 with a linear range of 15.6-500 ng/ml.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human CD40L at 10 µg/ml (100 µl/well) can bind human CD40 / Fc with a linear range of 7.8-125 ng/ml.
Binding
Measured by its binding ability in a functional ELISA. Immobilized human CD40L at 10 µg/ml (100 µl/well) can bind human CD40 with a linear range of 15.6-500 ng/ml.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human CD40L at 10 µg/ml (100 µl/well) can bind human CD40 / Fc with a linear range of 7.8-125 ng/ml.
Binding
Measured by its binding ability in a functional ELISA. Immobilized human CD40L at 10 µg/ml (100 µl/well) can bind human CD40 with a linear range of 15.6-500 ng/ml.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular CD40L Proteins
Source:
E. coli
Tag:
His, N-Terminal
Source:
E. coli
Tag:
6His, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/28/2024
Date Requested: 11/28/2024
