Target
CD28
Synonyms
CD28 | CD28 antigen | CD28 antigen (Tp44) | Tp44 | CD28 molecule
Modifications
Preservative Free.
Also available
Unmodified.
Conjugations
Unconjugated
Region
Extracellular domain of human CD28 fused to murine IgG2a Fc.
Expression System
CHO Cells
Source Species
Hamster-Chinese
Usage Summary
A soluble fusion protein consisting of the extracellular (134aa) domain of human CD28 fused to murine IgG2a Fc (233aa). CD28-muIg was tested for activity and quantitated in EIA. Fusion protein was captured using Goat-anti-mouse antibody coated plate. After 45 minute incubation, plates were washed twice and an excess of Mouse Ig was added to completely block the plate. Anti-CD28/Biotin (1 µg/ml) followed by Streptavidin/HRP, and TMB/H2O2 were used for detection. Fusion protein was detectable at 4 ng/ml in this assay.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl
Storage
Store at 2°C to 5°C for up to 1 year. Avoid freeze-thaw cycles. Open under aseptic conditions.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee