Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Location


Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers



Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-G47618-100 100 µg $372 
LS-G47618-1 1 mg $1,498 
Protein

Human CD274 / B7-H1 / PD-L1 Protein (Recombinant Mouse IgG1 Fc, C-terminus) (Met1-Thr239) - LS-G47618

Human CD274 / B7-H1 / PD-L1 Protein (Recombinant Mouse IgG1 Fc, C-terminus) (Met1-Thr239) - LS-G47618

Description:
CD274 / B7-H1 / PD-L1 Protein LS-G47618 is a Recombinant Human CD274 / B7-H1 / PD-L1 produced in HEK 293 Cells Met1-Thr239 with Mouse IgG1 Fc, C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Price
Catalog Number
$372
LS-G47618-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Description:
CD274 / B7-H1 / PD-L1 Protein LS-G47618 is a Recombinant Human CD274 / B7-H1 / PD-L1 produced in HEK 293 Cells Met1-Thr239 with Mouse IgG1 Fc, C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only

Specifications

Type
Recombinant Protein
Target
CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
  • Binding
  • ELISA
  • Functional Assay
  • Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
Mouse IgG1 Fc, C-terminus
Region
Met1-Thr239
Predicted Molecular Weight
The recombinant human CD274 consists of 455 amino acids and predicts a molecular mass of 51.7 kDa.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant Human PD1-His at 10 µg/ml (100 µl/well) can bind human PD-L1 with a linear range of 1.28-20 µg/ml. Measured by its ability to inhibit anti­CD3 antibody induced IL­2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.4-3 µg/ml.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from 20 mM Tris, 150 mM NaCl, 10 % Glycerol, pH 8.5, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024