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| Catalog Number | Size | Price |
|---|---|---|
| LS-G47614-100 | 100 µg | $441 |
| LS-G47614-1 | 1 mg | $2,261 |
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Human CD274 / B7-H1 / PD-L1 Protein (Recombinant) (Met1-Thr239) - LS-G47614
Human CD274 / B7-H1 / PD-L1 Protein (Recombinant) (Met1-Thr239) - LS-G47614
Description:
CD274 / B7-H1 / PD-L1 Protein LS-G47614 is a Recombinant Human CD274 / B7-H1 / PD-L1 produced in HEK 293 Cells Met1-Thr239. It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
CD274 / B7-H1 / PD-L1 Protein LS-G47614 is a Recombinant Human CD274 / B7-H1 / PD-L1 produced in HEK 293 Cells Met1-Thr239. It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Region
Met1-Thr239
Predicted Molecular Weight
The recombinant mature human B7-H1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 459 amino acids and predicts a molecular mass of 52 kDa. As a result of glycosylation, the rh B7-H1/Fc monomer migrates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized human PD-1 at 10 µg/ml (100 µl/well) can bind recombinant human B7-H1 / PD-L1 / Fc chimera with a linear range of 0.02-0.4 µg/ml.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD274 / B7-H1 / PD-L1
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Binding
Measured by its binding ability in a functional ELISA. Immobilized human PD-1 at 10 µg/ml (100 µl/well) can bind recombinant human B7-H1 / PD-L1 / Fc chimera with a linear range of 0.02-0.4 µg/ml.
Binding
Using the Octet RED System, the affinity constant (Kd) of PD-L1 Protein, Human, Recombinant (Fc Tag)(10084-H02H) bound to Atezolizuma was 0.5 µM.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human PD-1 at 10 µg/ml (100 µl/well) can bind recombinant human B7-H1 / PD-L1 / Fc chimera with a linear range of 0.02-0.4 µg/ml.
Binding
Using the Octet RED System, the affinity constant (Kd) of PD-L1 Protein, Human, Recombinant (Fc Tag)(10084-H02H) bound to Atezolizuma was 0.5 µM.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human PD-1 at 10 µg/ml (100 µl/well) can bind recombinant human B7-H1 / PD-L1 / Fc chimera with a linear range of 0.02-0.4 µg/ml.
Binding
Using the Octet RED System, the affinity constant (Kd) of PD-L1 Protein, Human, Recombinant (Fc Tag)(10084-H02H) bound to Atezolizuma was 0.5 µM.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human PD-1 at 10 µg/ml (100 µl/well) can bind recombinant human B7-H1 / PD-L1 / Fc chimera with a linear range of 0.02-0.4 µg/ml.
Binding
Using the Octet RED System, the affinity constant (Kd) of PD-L1 Protein, Human, Recombinant (Fc Tag)(10084-H02H) bound to Atezolizuma was 0.5 µM.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular CD274 / B7-H1 / PD-L1 Proteins
Source:
E. coli
Tag:
His-GST
Source:
Human
Tag:
6His, C-terminus
Source:
E. coli
Tag:
His
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
