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Catalog Number Size Price
LS-G47514-100 100 µg $441 
LS-G47514-1 1 mg $2,003 
CD274 / B7-H1 / PD-L1 Protein - Measured by its binding ability in a functional ELISA. Immobilized human B7-H1 at 20 µg/ml (100 µl/well) can bind human PD1 with a linear ranger of 0.032-0.8 µg/ml.
CD274 / B7-H1 / PD-L1 Protein
CD274 / B7-H1 / PD-L1 Protein - Measured by its binding ability in a functional ELISA. Immobilized human B7-H1 at 20 µg/ml (100 µl/well) can bind human PD1 with a linear ranger of 0.032-0.8 µg/ml.
CD274 / B7-H1 / PD-L1 Protein
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Human CD274 / B7-H1 / PD-L1 Protein (Recombinant His,C-terminus) (Met1-Thr239) - LS-G47514

Human CD274 / B7-H1 / PD-L1 Protein (Recombinant His,C-terminus) (Met1-Thr239) - LS-G47514

Description:
CD274 / B7-H1 / PD-L1 Protein LS-G47514 is a Recombinant Human CD274 / B7-H1 / PD-L1 produced in HEK 293 Cells Met1-Thr239 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Price
Catalog Number
$441
LS-G47514-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Description:
CD274 / B7-H1 / PD-L1 Protein LS-G47514 is a Recombinant Human CD274 / B7-H1 / PD-L1 produced in HEK 293 Cells Met1-Thr239 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only

Specifications

Type
Recombinant Protein
Target
CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
  • Binding
  • ELISA
  • Functional Assay
  • Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His,C-terminus
Region
Met1-Thr239
Predicted Molecular Weight
The recombinant mature human B7-H1 comprises 232 amino acids and predicts a molecular mass of 26.8 kDa. As a result of glycosylation, the rh B7-H1 migrates as an approximately 35-38 kDa protein in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 98 % as determined by SDS-PAGE
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized human B7-H1 at 20 µg/ml (100 µl/well) can bind human PD1 with a linear ranger of 0.032-0.8 µg/ml.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

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Binding

CD274 / B7-H1 / PD-L1 Protein - Measured by its binding ability in a functional ELISA. Immobilized human B7-H1 at 20 µg/ml (100 µl/well) can bind human PD1 with a linear ranger of 0.032-0.8 µg/ml.
Measured by its binding ability in a functional ELISA. Immobilized human B7-H1 at 20 µg/ml (100 µl/well) can bind human PD1 with a linear ranger of 0.032-0.8 µg/ml.

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

CD274 / B7-H1 / PD-L1 Protein

Binding

CD274 / B7-H1 / PD-L1 Protein - Measured by its binding ability in a functional ELISA. Immobilized human B7-H1 at 20 µg/ml (100 µl/well) can bind human PD1 with a linear ranger of 0.032-0.8 µg/ml.
Measured by its binding ability in a functional ELISA. Immobilized human B7-H1 at 20 µg/ml (100 µl/well) can bind human PD1 with a linear ranger of 0.032-0.8 µg/ml.

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

CD274 / B7-H1 / PD-L1 Protein

Binding

CD274 / B7-H1 / PD-L1 Protein - Measured by its binding ability in a functional ELISA. Immobilized human B7-H1 at 20 µg/ml (100 µl/well) can bind human PD1 with a linear ranger of 0.032-0.8 µg/ml.
Measured by its binding ability in a functional ELISA. Immobilized human B7-H1 at 20 µg/ml (100 µl/well) can bind human PD1 with a linear ranger of 0.032-0.8 µg/ml.

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

CD274 / B7-H1 / PD-L1 Protein

Binding

CD274 / B7-H1 / PD-L1 Protein - Measured by its binding ability in a functional ELISA. Immobilized human B7-H1 at 20 µg/ml (100 µl/well) can bind human PD1 with a linear ranger of 0.032-0.8 µg/ml.
Measured by its binding ability in a functional ELISA. Immobilized human B7-H1 at 20 µg/ml (100 µl/well) can bind human PD1 with a linear ranger of 0.032-0.8 µg/ml.

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

CD274 / B7-H1 / PD-L1 Protein

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024