Target
BMP7
Synonyms
BMP7 | Bone morphogenetic protein 7 | Eptotermin alfa | OP-1 | OP1 | Osteogenic protein 1 | BMP-7
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 117 amino acid recombinant protein has a predicted molecular mass of approximately 13 kD. Recombinant human BMP-7 is a 28.8 kD homodimeric glycoprotein. The predicted N-terminal amino acid is Met.
Expression System
CHO Cells
Source Species
Hamster-Chinese
Purification
Greater than 95% by SDS-PAGE and HPLC
Usage Summary
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA.
Bio-Activity
BMP-7 induces alkaline phosphatase production on ATDC-5 cells. The ED50 is equal to0.02 - 0.04 µg/ml, corresponding to a specific activity of 2.5 - 5.0 x 10^4 unit/mg.
Endotoxin
Less than 0.1 ng/µg of protein.
Storage
Store unopened at -20°C to -70°C. Store diluted at -20°C to -80°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee