Target
BMP4
Synonyms
BMP4 | BMP-2B | BMP-4 | BMP2B | BMP2B1 | Bone morphogenetic protein 2B | Bone morphogenetic protein 4 | DVR4 | MCOPS6 | OFC11 | Zyme
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 106 amino acid recombinant protein has a predicted molecular mass of approximately 12 kD. The DTT-non reduced protein migrates at approximately 31-36 kD on SDS-PAGE. The predicted N-terminal amino acid is Lys.
Purification
Greater than 97% by SDS-PAGE and HPLC
Usage Summary
Reconstitute 5 mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA.
Bio-Activity
BMP-4 induces alkaline phosphatase production on ATDC-5 cells. The expected ED50 for this effect is 5-10 ng/ml, corresponding to 1-2 x 10^5 unit/mg.
Endotoxin
Less than 0.1 ng/µg of protein.
Storage
Store unopened at -20°C to -70°C. Store diluted at -20°C to -80°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee