Target
APOA1 / Apolipoprotein A 1
Synonyms
APOA1 | ApoA-I | APOAI | Apolipoprotein A-I | Apolipoprotein A1 | Apo-AI
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The recombinant human APOA1/Fc is a disulfide-linked homodimeric protein.The reduced monomer consists of 481 amino acids migrates as approximately 55 kDa band in SDS-PAGE under reducing conditions as predicted.
Expression System
HEK 293 Cells
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized Human ApoAI at 10 µg/mL (100 µl/well) can bind biotinylated human SCARB1, The EC50 of biotinylated human SCARB1 is 0.37 µg/mL.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About APOA1 / Apolipoprotein A 1