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| Catalog Number | Size | Price |
|---|---|---|
| LS-B10456-100 | 100 µg (0.5 mg/ml) | $485 |
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PathPlus™ Polyclonal Goat anti‑Human TPO / Thyroid Peroxidase Antibody (aa850‑C‑term, IHC, WB) LS‑B10456
PathPlus™ Polyclonal Goat anti‑Human TPO / Thyroid Peroxidase Antibody (aa850‑C‑term, IHC, WB) LS‑B10456
Note: This antibody replaces LS-C154872
Antibody:
TPO / Thyroid Peroxidase Goat anti-Human Polyclonal (aa850-C-term) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
TPO / Thyroid Peroxidase Goat anti-Human Polyclonal (aa850-C-term) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Thyroid peroxidase (TPO) is a thyroid membrane-bound glycoprotein that functions in the iodination of tyrosine residues in thyroglobulin and pehnoxy-ester formation between iodinated tyrosines to generate thyroxine and triiodothyronine. Mutations in this gene are associated with autoimmune hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. The TPO antibody test is used to detect antibodies against TPO in patients with thyroid autoimmune disorders such as Graves or Hashimoto’s disease. TPO is expressed in thyroid follicular epithelium.
References: Endocr Rev 1992 13(2):192; ISRN Endocrinol 2013:509746
Target
Human TPO / Thyroid Peroxidase
Synonyms
TPO | MSA | Thyroid microsomal antigen | Thyroid peroxidase | TDH2A | TPX | Thyroperoxidase
Host
Goat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to residues in the C-Terminus of human TPO was used as an immunogen.
Epitope
aa850-C-term
Specificity
Human TPO / Thyroid peroxidase. This antibody is expected to recognize all reported isoforms (NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1).
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 110kD band observed in Human Thyroid Gland lysates (calculated MW of 103kD according to NP_000538.3). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TPO / Thyroid Peroxidase
Validation
Anti-TPO / Thyroid Peroxidase antibody IHC staining of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TPO / Thyroid Peroxidase antibody IHC staining of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
PathPlus™ TPO / Thyroid Peroxidase Antibody (aa850-C-term) for IHC, WB/Western LS-B10456 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
Western blot, ELISA
Species:
Mouse, Rat
Applications:
Western blot
Species:
Human
Applications:
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
