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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16242-50 | 50 µg (200 µg/ml) | $460 |
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PathPlus™ Recombinant Monoclonal Mouse anti‑Human GFAP Antibody (clone rASTRO/789, IHC, WB) LS‑B16242
PathPlus™ Recombinant Monoclonal Mouse anti‑Human GFAP Antibody (clone rASTRO/789, IHC, WB) LS‑B16242
Note: This antibody replaces LS-C751154
Antibody:
GFAP Mouse anti-Human Recombinant Monoclonal (rASTRO/789) Antibody
Application:
IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
GFAP Mouse anti-Human Recombinant Monoclonal (rASTRO/789) Antibody
Application:
IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
GFAP (glial fibrillary acidic protein) is an intermediate filament protein found in the central nervous system. Functionally, GFAP helps maintain cell shape and astrocyte mechanical strength, as well as participate in cell communication and blood brain barrier function. In immunohistochemistry, it is positive in astrocytes, ganglion cells and some ependymal cells of the central nervous system. In cancer, it is positive in ependymomas and in astrocytic tumors such as astrocytic gliomas, astrocytomas and glioblastomas.
References: Brain Research. 2007 July. 1158: 103–15. doi:10.1016/j.brainres.2007.04.070, PMID: 17555726; Neurology India. 2013. 61 (4): 383–8, PMID: 24005729; The Journal of Cell Biology. 1991 Mar. 112 (6): 1205–13. doi:10.1083/jcb.112.6.1205, PMID: 1999469; Neurosci Lett. 1996 May 3;209(1):29-32, PMID: 8734902.
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Recombinant Monoclonal
Clone
rASTRO/789
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified.
Also available Azide-free, Carrier-free.
Immunogen
Recombinant full-length human GFAP protein. Antigen Molecular Weight: ~50 kDa
Specificity
This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Applications
- IHC - Paraffin (1:50)
- Western blot (0.5 - 1 µg/ml)
- Flow Cytometry (1 - 2 µg/10E6 cells)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Western Blotting (0.5-1 ug/ml) - Immunohistology (Formalin-fixed - 0.25-0.5 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.
Presentation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C for up to 2 years.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GFAP
Validation
Human Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ GFAP Antibody (clone rASTRO/789) for IHC, WB/Western, Flow LS-B16242 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
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Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Rat, Bat, Bovine, Horse, Rabbit
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Pig, Human, Feline, Porcine
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Species:
Pig, Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Mouse, Human, Rat
Applications:
IHC, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC - Paraffin, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/18/2024
Date Requested: 11/18/2024
