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Catalog Number	Size	Price
LS-B2996-50	50 µg (0.5 mg/ml)	$485

FOXC1 Antibody - Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.

FOXC1 Antibody - Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.

FOXC1 Antibody - FOXC1 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

FOXC1 Antibody - FOXC1 antibody (0.5µg/ml) staining of transfected HEK293 transiently expressing full-length Human FOXC1 (myc and DYKDDDDK tagged). Detected by chemiluminescence.

FOXC1 Antibody - FOXC1 antibody (0.3µg/ml) staining of HEK293 (A) Nuclear MCF7 (B) and Negative control Daudi (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

FOXC1 Antibody - Antibody staining (0.5 ug/ml) of Human Bone Marrow lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

FOXC1 Antibody - FOXC1 antibody flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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PathPlus™ Polyclonal Goat anti‑Human FOXC1 Antibody (aa541‑553, IHC, IF, WB) LS‑B2996

Name: PathPlus™ FOXC1 Polyclonal Antibody Goat anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B2996-50
Price: 485.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

Neuroscience

(5.0) Rating

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Note: This antibody replaces LS-C54851, LS-C66329

Antibody:

FOXC1 Goat anti-Human Polyclonal (aa541-553) Antibody

Application:

IHC, IHC-P, IF, WB, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse, Xenopus, Zebrafish

Format:

Unconjugated, Unmodified

$485

LS-B2996-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

FOXC1 Goat anti-Human Polyclonal (aa541-553) Antibody

Application:

IHC, IHC-P, IF, WB, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse, Xenopus, Zebrafish

Format:

Unconjugated, Unmodified

Specifications

Description

FOXC1 is a forkhead transcription factor involved in embryogenesis, cardiac morphogenesis and ocular development. Inherited mutations in FOXC1 lead to glaucoma, Axenfeld-Rieger syndrome and cerebral small-vessel disease (CSVD), described by increased risk for stroke and progressive cognitive decline. Upregulation of FOXC1 in cancer is common and linked with poor prognosis, as it leads to increased expression of vimentin, N-cadherin and fibronectin and consequently promotes migration and metastasis. In immunohistochemistry, FOXC1 has highest cytoplasmic and nuclear positivity in the central nervous system and salivary gland, and is also moderately expressed in most other tissues throughout the body. References: Developmental biology. 2006. 421-436. ISBN-10: 0-87893-243-7; Molecular Medicine Reports. 12 (6): 8003–9, PMID: 26461269; Oncogene. 2017 Jul 13;36(28):3957-3963, PMID: 28288141; J Clin Invest. 2014 Nov;124(11):4877-81, PMID: 25250569

Target

Human FOXC1

Synonyms

See All FOXC1 Antibodies

Host

Goat

Reactivity

Human, Monkey, Mouse, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence RTSGAFVYDCSKF, from the C-Terminus of protein sequence according to NP_001444.2.

Epitope

aa541-553

Specificity

Human FOXC1.

Applications

IHC
IHC - Paraffin (3.75 µg/ml)
Immunofluorescence (10 µg/ml)
Western blot (0.5 - 1 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:32000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B2996 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2996 was determined to be 3.75 ug/ml.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About FOXC1

Uniprot: Q12948 NCBI: NM_001453 NP_001444.2

Validation

Anti-FOXC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.

Anti-FOXC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.

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PathPlus™ Validation Process