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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16294-100 | 100 µg (200 mg/ml) | $525 |
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PathPlus™ Recombinant Monoclonal Rabbit anti‑Human CD45 / LCA Antibody (clone PTPRC/1783R, IHC, IF, WB) LS‑B16294
PathPlus™ Recombinant Monoclonal Rabbit anti‑Human CD45 / LCA Antibody (clone PTPRC/1783R, IHC, IF, WB) LS‑B16294
Note: This antibody replaces LS-C751329
Antibody:
CD45 / LCA Rabbit anti-Human Recombinant Monoclonal (PTPRC/1783R) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CD45 / LCA Rabbit anti-Human Recombinant Monoclonal (PTPRC/1783R) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
CD45 (LCA, PTPRC) is a type 1 transmembrane protein tyrosine phosphatase that is a marker for leukocytes. It is expressed on almost all hematopoietic cells (B and T-lymphocytes, granulocytes, monocytes, mast cells, dendritic cells, and macrophages). It is positive in many hematolymphoid neoplasms and may assist in the classification of lymphomas and leukemias. It is negative in mature erythrocytes, platelets, plasma cells, and mature megakaryocytes. Defects in PTPRC are one cause of severe combined immunodeficiency syndrome characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.
References: Hermiston et al. Annual review of immunology 21.1 (2003): 107-137, DOI: 10.1146/annurev.immunol.21.120601.140946;Am J Clin Pathol 1998;110:797, PMID: 9844593
Target
Human CD45 / LCA
Synonyms
PTPRC | B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Recombinant Monoclonal
Clone
PTPRC/1783R
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified.
Also available Azide-free, Carrier-free.
Immunogen
Recombinant full-length human CD45RB protein. Antigen Molecular Weight: 180-220 kDa
Specificity
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.
Applications
- IHC - Paraffin (1:200)
- Immunofluorescence (1 - 2 µg/ml)
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (1 - 2 µg/10E6 cells)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistology (Formalin-fixed - 0.5-1.0 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.
Presentation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD45 / LCA
Validation
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ CD45 / LCA Antibody (clone PTPRC/1783R) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B16294 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human, Monkey, Mouse, Rat, Dog
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/15/2024
Date Requested: 11/15/2024
