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Catalog Number	Size	Price
LS-B16619-100	100 µg (0.5 mg/ml)	$485

c-Kit / CD117 Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)

c-Kit / CD117 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

c-Kit / CD117 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

c-Kit / CD117 Antibody - c-Kit / CD117 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

c-Kit / CD117 Antibody - c-Kit / CD117 antibody immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

c-Kit / CD117 Antibody - c-Kit / CD117 antibody (1µg/ml) staining of Human Hippocampus lysate (A) + peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.

c-Kit / CD117 Antibody - KIT antibody (1 ug/ml) staining of Human Hippocampus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

c-Kit / CD117 Antibody - c-Kit / CD117 antibody flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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PathPlus™ Polyclonal Goat anti‑Human c‑Kit / CD117 Antibody (aa685‑697, IHC, IF, WB) LS‑B16619

Name: PathPlus™ c-Kit / CD117 Polyclonal Antibody Goat anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B16619-100
Price: 485.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

Cancer Pathology Cancer

(5.0) Rating

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Note: This antibody replaces LS-C205047

Antibody:

c-Kit / CD117 Goat anti-Human Polyclonal (aa685-697) Antibody

Application:

IHC-P, IF, WB, Flo, Peptide-ELISA

Reactivity:

Human, Bovine, Dog, Pig

Format:

Unconjugated, Unmodified

$485

LS-B16619-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

c-Kit / CD117 Goat anti-Human Polyclonal (aa685-697) Antibody

Application:

IHC-P, IF, WB, Flo, Peptide-ELISA

Reactivity:

Human, Bovine, Dog, Pig

Format:

Unconjugated, Unmodified

Specifications

Description

CD117 (mast/stem cell growth factor receptor, SCFR, KIT oncogene) is a protein tyrosine kinase receptor for the cytokine KITLG/SCF. Activation regulates apoptosis and cell proliferation, differentiation, chemotaxis, and adhesion. Altered forms are associated with cancer. CD117 is expressed on hematopoietic stem cells and progenitor cells and some nonhematopoietic cells such as mast cells, melanocytes, germ cells, interstitial cells of Cajal, skin adnexa epithelial cells, and some cerebellar neurons and in breast tissue. It is also expressed in tumors of these cell types. Staining is used primarily to identify gastrointestinal stromal tumors and also small-cell lung carcinoma, acute myelogenous leukemia, chronic myelogenous leukemia, breast carcinoma, glioblastoma, and melanoma. References: Int J Biochem Cell Biol. 2007;39(11):1995-1998. doi: 10.1016/j.biocel.2006.12.005; Appl Immunohistochem Mol Morphol. 2005;13(3):205-220. PMID: 16082245; Oncogene. 2005;24(26):4271-4280. doi: 10.1038/sj.onc.1208587; Hum Pathol. 2002;33(12):1182-1187. doi: 10.1053/hupa.2002.129199

Target

Human c-Kit / CD117

Synonyms

See All c-Kit / CD117 Antibodies

Host

Goat

Reactivity

Human, Bovine, Dog, Pig (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence KRDSFICSKQEDH, from the internal region of the protein sequence according to NP_000213.1NP_001087241.1.

Epitope

aa685-697

Specificity

Human c-Kit / CD117. This antibody is expected to recognize both reported isoforms (NP_000213.1; NP_001087241.1).

Applications

IHC - Paraffin (10 µg/ml)
Immunofluorescence (10 µg/ml)
Western blot (1 - 3 µg/ml)
Flow Cytometry
Peptide Enzyme-Linked Immunosorbent Assay (1:128000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 110kD band observed in Human Brain (Hippocampus) lysates (calculated MW of 110kD according to NP_000213.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 35kD. This band was successfully blocked by incubation with the immunizing peptide.

Presentation

0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About c-Kit / CD117

Uniprot: P10721 NCBI: NM_000222 NP_000213.1