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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16231-100 | 100 µg (200 µg/ml) | $525 |
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PathPlus™ Recombinant Monoclonal Rabbit anti‑Human BCL2 / Bcl‑2 Antibody (clone BCL2/2210R, IHC, WB) LS‑B16231
PathPlus™ Recombinant Monoclonal Rabbit anti‑Human BCL2 / Bcl‑2 Antibody (clone BCL2/2210R, IHC, WB) LS‑B16231
Note: This antibody replaces LS-C751342
Antibody:
BCL2 / Bcl-2 Rabbit anti-Human Recombinant Monoclonal (BCL2/2210R) Antibody
Application:
IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
BCL2 / Bcl-2 Rabbit anti-Human Recombinant Monoclonal (BCL2/2210R) Antibody
Application:
IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
BCL2 is a regulator of apoptosis that functions to promote cell survival and inhibit the actions of proteins involved in the apoptosis cascade. Overexpression of BCL2 has an oncogenic effect and is correlated with increasing the lifespan of B cells. Cancers that are positive include follicular lymphomas due to t(14,18)(q32;q21), which brings BCL2 gene adjacent to the active immunoglobulin heavy chain (IgH) gene. In addition, positive staining is expected in diffuse large B-cell cell lymphomas and myelodysplastic syndrome. In normal tissues, positive expression is found in mantle zone B cells, germinal center T cells, and several other tissues.
References: Cell Death and Differentiation 25:65, 2018; Am J Surg Pathol 2011;35:1691, Am J Surg Pathol 2009;33:591
Target
Human BCL2 / Bcl-2
Synonyms
BCL2 | Apoptosis regulator Bcl-2 | B-cell CLL/lymphoma 2 | B-cell lymphoma protein 2 | Bcl-2 | PPP1R50
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Recombinant Monoclonal
Clone
BCL2/2210R
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified.
Also available Azide-free, Carrier-free.
Immunogen
Recombinant full-length human bcl-2 protein. Antigen Molecular Weight: 25-26 kDa
Specificity
This antibody recognizes a protein of 25-26 kDa , identified as the bcl-2alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Applications
- IHC - Paraffin (1:100)
- Western blot (1 - 2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Western Blotting (0.5-1 ug/ml) - Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA Buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.
Presentation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C for up to 2 years.
Restrictions
For research use only. Intended for use by laboratory professionals.
About BCL2 / Bcl-2
Validation
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ BCL2 / Bcl-2 Antibody (clone BCL2/2210R) for IHC, WB/Western LS-B16231 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
