Human EPCAM

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

MOC-31

Unconjugated

Tissue culture supernatant

Concentrated

This antibody is reactive with lung cancer associated antigens and has been studied and categorized in different clusters of reactivity patterns during the First International Workshop on Small Cell Lung Cancer Antigens held in London in April 1987. MOC-31 reacts with most epithelia, and, in lung cancer, all lung carcinomas. The membrane-associated proteins detected by MOC-31 appear to have an apparent molecular weight of 35-40 kD. MOC-31 is not reactive with normal and malignant mesothelia and therefore is especially useful for the detection of carcinoma cells in ascites or pleural effusions (4).

• IHC
• IHC - Paraffin (1:100)
• IHC - Frozen
• Western blot

The antibody is useful in immunohistochemistry and immunoblotting. MOC-31 react with antigens detectable in cryostat section. For use on frozen tissue and paraffin tissue after antigen retrieval. Using the antigen retrieval techniques this antibody discriminates between cells which originate from mesothelium and epithelium. General procedure to perform cryostat sectioning and immunostaining: Cryostat sectioning and immunostaining is done as described by de Leij et al. Sections (about 6 micron thick) are cut in a cryostat at -20°C and placed on glass slides. After drying at RT and fixation in acetone (water-free), the sections are washed 2-3 times in PBS. Subsequently (after drying the glass area around the sections) 25 ul of undiluted monoclonal antibody preparation is applied to the wet sections. After incubation for 45 mins. in a humidified atmosphere, the sections are washed again in PBS (2-3 times) and the second step reagent (an appropriately diluted HRPO-conjugated, anti mouse Ig preparation, supplemented with 1% human serum) is applied to the wet sections. After incubation for an additional 20 mins., the sections are washed again in PBS (2-3) times and the staining reaction is performed with 3-aminoethylcarbazole (10 mg dissolved in 2.5 ml dimethylformamide, subsequently 0.05 M acetate buffer, pH 4.9, is added to a total volume of 50 ml, after which the solution is filtrated and H2O2 is added to a final concentration of about 0.03%). A positive reaction is indicated by red deposit. The nuclei of the cells present in the sections are counterstained with Mayer's hematoxylin to obtain a good histological picture. Working dilution: approx 1:20.

Tissue culture supernatant (8% FBS), <0.1% sodium azide

Store at 2-8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16422 NCBI: NM_002354 NP_002345.2