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Catalog Number Size Price
LS-K246-100 100 Tests $585 
NAD + NADH Assay Kit

NAD/NADH Assay Kit (Colorimetric) - LS-K246

NAD/NADH Assay Kit (Colorimetric) - LS-K246

Available for shipment within the USA only
Description:
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NAD+/NADH concentration are very desirable. This NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH and with minimal interference (<1%) by NADP+/NADPH. Our assay is a convenient Method to measure NAD, NADH and their ratio.
Price
Catalog Number
$585
LS-K246-100

Available for USA Shipment Only
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Description:
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NAD+/NADH concentration are very desirable. This NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH and with minimal interference (<1%) by NADP+/NADPH. Our assay is a convenient Method to measure NAD, NADH and their ratio.

Specifications

Name
NAD/NADH Assay Kit (Colorimetric)
Type
Detection/Quantition
Usage
For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.
Target
NAD + NADH
SampleType
Cell Extracts, Tissue Extracts
Detection
Colorimetric (565 nm Absorbance)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer
  • Enzyme A
  • Lactate
  • Enzyme B
  • MTT Solution
  • NAD (P)/NAD (P)H Extraction Buffer
  • NAD Standard
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (visible)
Equipment
Microplate spectrophotometer (visible)
Conditions
Shipped -20°C Dry Ice, Store at -20°C, 6 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Colorimetry

NAD + NADH Assay Kit

Colorimetry

NAD + NADH Assay Kit

Colorimetry

NAD + NADH Assay Kit

Colorimetry

NAD + NADH Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/29/2024