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Antibodies > ZIF268 / EGR1 > LS-C662514
 
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Catalog Number Size Price
LS-C662514-10 10 µg $318 
LS-C662514-100 100 µg $470 
ZIF268 / EGR1 Antibody - Western blot analysis of Egr1 using anti-Egr1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates, Lane 2: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Egr1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Egr1 at approximately 75KD. The expected band size for Egr1 is at 58KD.
ZIF268 / EGR1 Antibody - Western blot - Anti-Egr1 Picoband antibody
ZIF268 / EGR1 Antibody - Western blot analysis of Egr1 using anti-Egr1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates, Lane 2: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Egr1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Egr1 at approximately 75KD. The expected band size for Egr1 is at 58KD.
ZIF268 / EGR1 Antibody - Western blot - Anti-Egr1 Picoband antibody
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Polyclonal Rabbit anti‑Human ZIF268 / EGR1 Antibody (WB) LS‑C662514

Polyclonal Rabbit anti‑Human ZIF268 / EGR1 Antibody (WB) LS‑C662514

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Antibody:
ZIF268 / EGR1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662514-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ZIF268 / EGR1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
EGR1 antibody LS-C662514 is an unconjugated rabbit polyclonal antibody to human EGR1 (ZIF268). Validated for ELISA and WB.
Target
Human ZIF268 / EGR1
Synonyms
EGR1 | AT225 | Early growth response 1 | G0S30 | KROX24 | NGFIA | KROX-24 | TIS8 | ZIF-268 | ZIF268 | Zinc finger protein Krox-24 | EGR-1 | NGFI-A | Transcription factor ETR103 | Transcription factor Zif268 | Zinc finger protein 225
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived human Egr1 recombinant protein (Position: A239-R327).
Specificity
Detected in neutrophils (at protein level).
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ZIF268 / EGR1
P18146 NM_001964 NP_001955.1

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Applications: IHC, IHC - Paraffin, Western blot, ELISA
ZIF268 / EGR1 Antibody - Immunofluorescence of monoclonal antibody to EGR1 on HeLa cell (antibody concentration 10 ug/ml).
ZIF268 / EGR1 Antibody (clone 6E8) LS‑C104940
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Applications: Immunofluorescence, Western blot, ELISA
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ZIF268 / EGR1 Antibody - NIH 3T3 Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ZIF268 / EGR1 Antibody (aa53‑102) LS‑C31244
Species: Mouse, Frog
Applications: Western blot
ZIF268 / EGR1 Antibody - Confocal immunofluorescence of EGR1 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
ZIF268 / EGR1 Antibody (aa9‑37) LS‑C167664
Species: Human, Mouse
Applications: Immunofluorescence, Western blot, Flow Cytometry
ZIF268 / EGR1 Antibody - Immunohistochemical analysis of EGR1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ZIF268 / EGR1 Antibody (C‑Terminus) LS‑C352150
Species: Human, Mouse, Rat, Bovine, Chicken
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
See all ZIF268 / EGR1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/12/2025
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