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Antibodies > ZAP70 > LS-C801101
 
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Catalog Number Size Price
LS-C801101-100 100 µl (1 mg/ml) $379 
LS-C801101-200 200 µl (1 mg/ml) $421 
ZAP70 Antibody - 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22° C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ZAP70 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
ZAP70 Antibody - Western blot analysis of ZAP-70 phosphorylation expression in Jurkat whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ZAP70 Antibody - Western blot analysis of Phospho-ZAP-70 (Tyr493) expression in various lysates
ZAP70 Antibody - 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22° C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ZAP70 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
ZAP70 Antibody - Western blot analysis of ZAP-70 phosphorylation expression in Jurkat whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ZAP70 Antibody - Western blot analysis of Phospho-ZAP-70 (Tyr493) expression in various lysates
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Polyclonal Rabbit anti‑Human ZAP70 Antibody (phospho‑Tyr493, IHC, IF, WB) LS‑C801101

Polyclonal Rabbit anti‑Human ZAP70 Antibody (phospho‑Tyr493, IHC, IF, WB) LS‑C801101

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Antibody:
ZAP70 Rabbit anti-Human Polyclonal (pTyr493) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801101-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ZAP70 Rabbit anti-Human Polyclonal (pTyr493) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ZAP70 antibody LS-C801101 is an unconjugated rabbit polyclonal antibody to ZAP70 (pTyr493) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human ZAP70
Synonyms
ZAP70 | 70 kDa zeta-associated protein | SRK | STCD | STD | Syk-related tyrosine kinase | Tyrosine-protein kinase ZAP-70 | TZK | ZAP-70 | Zeta-associated protein 70
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyrosine 493.
Epitope
pTyr493
Specificity
Phospho-ZAP-70 (Tyr493) Antibody detects endogenous levels of ZAP-70 only when phosphorylated at Tyrosine 493.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ZAP70
P43403 NM_207519 P43403

Publications (0)

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Customer Reviews (0)

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Featured Products

ZAP70 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ZAP-70 (Phospho-Tyr493) Antibody. The picture on the right is blocked with the phospho peptide.
ZAP70 Antibody (phospho‑Tyr493) LS‑C117511
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
ZAP70 Antibody - Immunohistochemical analysis of ZAP70 (pY319) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ZAP70 Antibody (phospho‑Tyr319) LS‑C413113
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
ZAP70 Antibody - Immunohistochemistry analysis of paraffin-embedded human lymph node, using ZAP-70 (Phospho-Tyr315) Antibody. The picture on the right is blocked with the phospho peptide.
ZAP70 Antibody (phospho‑Tyr315) LS‑C199744
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
ZAP70 Antibody - ZAP70 antibody Western Blot of Mouse Kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ZAP70 Antibody (aa301‑350) LS‑C151431
Species: Mouse
Applications: Western blot
ZAP70 ELISA Kit
Mouse/Human Phospho-ZAP70 (Tyr315) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1238
Reactivity: Human, Mouse
Range: Positive/Negative
ZAP70 ELISA Kit
Mouse/Human/Rat Phospho-ZAP70 (Tyr493) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1650
Reactivity: Human, Mouse, Rat
Range: Positive/Negative
See all ZAP70 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
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