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Catalog Number	Size	Price
LS-C413113-100	100 µl (1 mg/ml)	$341

ZAP70 Antibody - Immunohistochemical analysis of ZAP70 (pY319) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

ZAP70 Antibody - Immunofluorescent analysis of ZAP70 (pY319) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

ZAP70 Antibody - Western blot analysis of ZAP70 (pY319) expression in SHSY5Y (A); Jurkat (B) whole cell lysates.

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Polyclonal Rabbit anti‑Human ZAP70 Antibody (phospho‑Tyr319, IHC, IF, WB) LS‑C413113

Name: Anti-ZAP70 Antibody | Rabbit anti-Human Polyclonal ICC,IF,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C413113-100
Price: 341.0000 USD
Availability: InStock

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Antibody:

ZAP70 Rabbit anti-Human Polyclonal (pTyr319) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$341

LS-C413113-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ZAP70 Rabbit anti-Human Polyclonal (pTyr319) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

ZAP70 antibody LS-C413113 is an unconjugated rabbit polyclonal antibody to ZAP70 (pTyr319) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB.

Target

Human ZAP70

Synonyms

ZAP70 | 70 kDa zeta-associated protein | SRK | STCD | STD | Syk-related tyrosine kinase | Tyrosine-protein kinase ZAP-70 | TZK | ZAP-70 | Zeta-associated protein 70

See All ZAP70 Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the central region of human ZAP70 (pY319).

Epitope

pTyr319

Specificity

Recognizes endogenous levels of ZAP70 (pY319) protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence (1:100 - 1:500)
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ZAP70

Uniprot: P43403 NCBI: NM_207519 P43403