Target
Human ZAP70
Synonyms
ZAP70 | 70 kDa zeta-associated protein | SRK | STCD | STD | Syk-related tyrosine kinase | Tyrosine-protein kinase ZAP-70 | TZK | ZAP-70 | Zeta-associated protein 70
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic phospho-peptide (KLH coupled) corresponding to residues surrounding Tyr319 of human Zap-70.
Specificity
Detects endogenous levels of human Zap-70 only when phosphorylated at Tyr319. Cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352. Species cross-reactivity: Human and mouse based on 100% sequence homology.
Applications
- ICC
- Immunofluorescence
- Western blot (1:1000)
- Immunoprecipitation (1:100)
- Flow Cytometry (1:25)
Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry. Western Blot: 1:1000. Immunoprecipitation: 1:100. Immunocytochemistry (for IF): 1:100. Flow Cytometry: 1:25.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee