Target
Human YES1 / c-Yes
Synonyms
YES1 | C-yes | Cyes | Cellular yes-1 protein | HsT441 | Oncogene kinase yes1 | p61-YES | p61yes | p62c-yes | Proto-oncogene c-Yes | Tyrosine-protein kinase Yes | Yes kinase | v-yes | Yes | Yamaguchi sarcoma oncogene
Reactivity
Human, Horse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human Yes1 (7-35 aa KENKSPAIKYRPENTPEPVSTSVSHYGAE), different from the related mouse sequence by six amino acids, and from the related rat sequence by seven amino acids.
Specificity
Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes in the basal epidermal layer of epidermis. .
Applications
- IHC
- IHC - Paraffin (0.5 - 1 µg/ml)
- Western blot (0.1 - 0.5 µg/ml)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
WB: The detection limit for Yes1 is approximately 0.1 ng/lane under reducing conditions. IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee