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Catalog Number	Size	Price
LS-C358884-30	30 µl (1 mg/ml)	$263
LS-C358884-100	100 µl (1 mg/ml)	$315
LS-C358884-200	200 µl (1 mg/ml)	$379

WAS / WASP Antibody - Immunohistochemical analysis of WASP (pY290) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

WAS / WASP Antibody - Immunofluorescent analysis of WASP (pY290) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

WAS / WASP Antibody - Western blot analysis of WASP (pY290) expression in Jurkat (A); TF1 (B) whole cell lysates.

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Polyclonal Rabbit anti‑Human WAS / WASP Antibody (phospho‑Tyr290, IHC, IF, WB) LS‑C358884

Name: Anti-WAS / WASP Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C358884-30
Price: 263.0000 USD
Availability: InStock

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Antibody:

WAS / WASP Rabbit anti-Human Polyclonal (pTyr290) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, Flo

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$263

LS-C358884-30

$315

LS-C358884-100

$379

LS-C358884-200

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(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

WAS / WASP Rabbit anti-Human Polyclonal (pTyr290) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, Flo

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

WASP antibody LS-C358884 is an unconjugated rabbit polyclonal antibody to WASP (WAS) (pTyr290) from human. It is reactive with human, mouse and rat. Validated for Flow, ICC, IF, IHC and WB.

Target

Human WAS / WASP

Synonyms

WAS | Eczema-thrombocytopenia | IMD2 | SCNX | WASP | THC | THC1 | Wiskott-Aldrich syndrome | Thrombocytopenia 1 (X-linked)

See All WAS / WASP Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the central region of human WASP (pY290).

Epitope

pTyr290

Specificity

Recognizes endogenous levels of WASP (pY290) protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence (1:100 - 1:500)
Western blot (1:500 - 1:1000)
Flow Cytometry (1:100 - 1:200)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About WAS / WASP

Uniprot: P42768 NCBI: NM_000377 NP_000368.1