Target
Vesicular Stomatitis Virus VSV-g Tag
Reactivity
Vesicular Stomatitis Virus
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Immunogen
Synthetic peptide (AA 497-511) from the C-Terminus of VSV, Vesicular Stomatitis Virus glycoprotein(KLH coupled).
Specificity
Recognizes native and denatured VSV containing proteins. Recognizes immature form of the VSV-G localized in the RER and in the cisternae of Golgi complex. Recognizes the mature VSV-G at the surface and in the budding virus. Does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain.
Applications
- Western blot (1:1000 - 1:10000)
- ELISA (1:5000 - 1:20000)
Usage
Suitable for use in ELISA and Western Blot. ELISA: 1:5000-1:20000 (50-100 ng antigen/well). Western Blot: 1:1000-1:10000 (chemiluminescence). Detects native and denatured VSV proteins.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee