Target
Vesicular Stomatitis Virus VSV-g Tag
Reactivity
Vesicular Stomatitis Virus
(tested or 100% immunogen sequence identity)
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxy terminal of vesicular stomatitis virus glycoprotein (VSV-G).
Specificity
VSV-G protein
Applications
- IHC (1:1000 - 1:5000)
- Western blot (1:2000 - 1:10000)
- ELISA (1:10000 - 1:50000)
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Usage
This affinity purified antibody has been tested for use in ELISA and by immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. For standard indirect immunofluor-escence assays, we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200. At higher concentrations, a filamentous background staining may be present. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Restore with 1.0 mL of deionized water (or equivalent).
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee