Target
Human UMOD / Uromodulin
Synonyms
UMOD | ADMCKD2 | FJHN | HNFJ | MCKD2 | THGP | THP | Uromucoid | Tamm-Horsfall glycoprotein | HNFJ1 | Uromodulin
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence DRCPHTRDSTIQ, from the internal region of the protein sequence according to NP_003352.2.
Specificity
Human UMOD / Uromodulin. Reported variants represent identical protein: NP_001008390.1, NP_003352.2
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 80kD band observed in lysates of cell line HeLa (calculated MW of 69.8kD according to NP_003352.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Williams et al, Hum Mol Genet. 2009 Aug 15;18(16):2963-74. PMID: 19465746). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee