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Catalog Number Size Price
LS-C157021-400 400 µl $393 
ULK1 Antibody - Fluorescent image of U251 cells stained with ULK1 (phospho S556) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ULK1 (phospho S556) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ULK1 (phospho S556) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
ULK1 Antibody - Dot blot of ULK1 Antibody (Phospho S556) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
ULK1 Antibody - Fluorescent image of U251 cells stained with ULK1 (phospho S556) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ULK1 (phospho S556) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ULK1 (phospho S556) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
ULK1 Antibody - Dot blot of ULK1 Antibody (Phospho S556) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
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Polyclonal Rabbit anti‑Human ULK1 Antibody (phospho‑Ser556, IF) LS‑C157021

Polyclonal Rabbit anti‑Human ULK1 Antibody (phospho‑Ser556, IF) LS‑C157021

Antibody:
ULK1 Rabbit anti-Human Polyclonal (pSer556) Antibody
Application:
IF, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C157021-400
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ULK1 Rabbit anti-Human Polyclonal (pSer556) Antibody
Application:
IF, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ULK1 antibody LS-C157021 is an unconjugated rabbit polyclonal antibody to human ULK1 (pSer556). Validated for DB and IF.
Target
Human ULK1
Synonyms
ULK1 | ATG1A | ATG1 | HATG1 | KIAA0722 | Unc-51-like kinase 1 | Unc51.1 | UNC51
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
pSer556
Specificity
This ULK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S556 of human ULK1.
Applications
  • Immunofluorescence (1:200)
  • Dot Blot (1:500)
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ULK1
O75385 NM_003565 NP_003556.1

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Requested From: United States
Date Requested: 11/29/2024