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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3386-200 | 200 µl (0.5 mg/ml) | $460 |
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2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human UBC / Ubiquitin C Antibody (aa1‑32, IHC, WB) LS‑B3386
IHC‑plus™ Polyclonal Rabbit anti‑Human UBC / Ubiquitin C Antibody (aa1‑32, IHC, WB) LS‑B3386
Note: This antibody replaces LS-C98776
Antibody:
UBC / Ubiquitin C Rabbit anti-Human Polyclonal (aa1-32) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
UBC / Ubiquitin C Rabbit anti-Human Polyclonal (aa1-32) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Ubiquitin C antibody LS-B3386 is an unconjugated rabbit polyclonal antibody to human Ubiquitin C (UBC) (aa1-32). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human UBC / Ubiquitin C
Synonyms
UBC | HUBCEP52 | L40 | HMG20 | RPL40 | Ubiquitin | Polyubiquitin-C | CEP52 | Ubiquitin C
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Hamster, Pig, Rabbit, Chicken, Xenopus, Drosophila, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa1-32
Specificity
This Ubiquitin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-32 amino acids from the N-terminal region of human Ubiquitin.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1:1000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3386 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3386 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About UBC / Ubiquitin C
Validation
Anti-UBC / Ubiquitin C antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-UBC / Ubiquitin C antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ UBC / Ubiquitin C Antibody (aa1-32) for IHC, WB/Western LS-B3386 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Protein aggregates are recruited to aggresome by histone deacetylase 6 via unanchored ubiquitin C termini. Ouyang H, Ali YO, Ravichandran M, Dong A, Qiu W, MacKenzie F, Dhe-Paganon S, Arrowsmith CH, Zhai RG. The Journal of biological chemistry. 2012 287:2317-27.
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Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Reactivity:
Human
Range:
0.16-10 ng/ml
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/2/2024
Date Requested: 11/2/2024
