Human Tyrosinase

Mouse

Human, Cat, Dog (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Azide-free

Recombinant tyrosinase protein. Epitope: 1-433. Cellular Localization: Cytoplasmic.

Recognizes a cluster of proteins between 70-80kD, identified as tyrosinase. Occasionally a minor band at 55kD is also detected. Does not cross-react with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Species cross-reactivity: Human, canine and feline. Weak cross-reactivity with mouse.

• IHC
• IHC - Paraffin
• ICC
• Immunofluorescence
• Western blot (1 - 2 µg/ml)

Suitable for use in Immunocytology, Immunofluorescence, Western Blot and Immunohistology. Western Blot: 1-2 ug/ml for 2 hours at RT. Immunohistology: 2-4 ug/ml for 30 minutes at RT (formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Melanoma cell lines (SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37), melanocytes in skin and Melanomas (1). The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS, pH 7.4. No preservatives or stabilizing proteins added.

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14679 NCBI: NM_000372 NP_000363.1