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Antibodies > TXN / Thioredoxin / TRX > LS-C756461
 
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Catalog Number Size Price
LS-C756461-10 10 µg $318 
LS-C756461-100 100 µg $470 
TXN / Thioredoxin / TRX Antibody - IHC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody. Thioredoxin TRX was detected in immunocytochemical section of LLC cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Thioredoxin TRX Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
TXN / Thioredoxin / TRX Antibody - IHC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody. Thioredoxin TRX was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Thioredoxin TRX Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
TXN / Thioredoxin / TRX Antibody - IHC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody. Thioredoxin TRX was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Thioredoxin TRX Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
TXN / Thioredoxin / TRX Antibody - IF analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody Thioredoxin TRX was detected in immunocytochemical section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Thioredoxin TRX Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
TXN / Thioredoxin / TRX Antibody - Western blot analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates,Lane 2: rat kidney tissue lysates,Lane 3: rat small intestine tissue lysates,Lane 4: rat spleen tissue lysates,Lane 5: rat stomach tissue lysates,Lane 6: rat lung tissue lysates,Lane 7: rat smooth muscle tissue lysates,Lane 8: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thioredoxin TRX antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Thioredoxin TRX at approximately 12KD. The expected band size for Thioredoxin TRX is at 12KD.
TXN / Thioredoxin / TRX Antibody - Western blot analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates,Lane 2: mouse kidney tissue lysates,Lane 3: mouse small intestine tissue lysates,Lane 4: mouse spleen tissue lysates,Lane 5: mouse stomach tissue lysates,Lane 6: mouse lung tissue lysates,Lane 7: mouse smooth muscle tissue lysates,Lane 8: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thioredoxin TRX antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Thioredoxin TRX at approximately 12KD. The expected band size for Thioredoxin TRX is at 12KD.
TXN / Thioredoxin / TRX Antibody - IHC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody. Thioredoxin TRX was detected in immunocytochemical section of LLC cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Thioredoxin TRX Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
TXN / Thioredoxin / TRX Antibody - IHC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody. Thioredoxin TRX was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Thioredoxin TRX Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
TXN / Thioredoxin / TRX Antibody - IHC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody. Thioredoxin TRX was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Thioredoxin TRX Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
TXN / Thioredoxin / TRX Antibody - IF analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody Thioredoxin TRX was detected in immunocytochemical section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Thioredoxin TRX Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
TXN / Thioredoxin / TRX Antibody - Western blot analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates,Lane 2: rat kidney tissue lysates,Lane 3: rat small intestine tissue lysates,Lane 4: rat spleen tissue lysates,Lane 5: rat stomach tissue lysates,Lane 6: rat lung tissue lysates,Lane 7: rat smooth muscle tissue lysates,Lane 8: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thioredoxin TRX antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Thioredoxin TRX at approximately 12KD. The expected band size for Thioredoxin TRX is at 12KD.
TXN / Thioredoxin / TRX Antibody - Western blot analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates,Lane 2: mouse kidney tissue lysates,Lane 3: mouse small intestine tissue lysates,Lane 4: mouse spleen tissue lysates,Lane 5: mouse stomach tissue lysates,Lane 6: mouse lung tissue lysates,Lane 7: mouse smooth muscle tissue lysates,Lane 8: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thioredoxin TRX antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Thioredoxin TRX at approximately 12KD. The expected band size for Thioredoxin TRX is at 12KD.
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Polyclonal Rabbit anti‑Mouse TXN / Thioredoxin / TRX Antibody (IHC, WB) LS‑C756461

Polyclonal Rabbit anti‑Mouse TXN / Thioredoxin / TRX Antibody (IHC, WB) LS‑C756461

Submit Review LSBio Guarantee
Antibody:
TXN / Thioredoxin / TRX Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC-P, ICC, WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756461-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TXN / Thioredoxin / TRX Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC-P, ICC, WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
TRX antibody LS-C756461 is an unconjugated rabbit polyclonal antibody to TRX (TXN / Thioredoxin) from mouse. It is reactive with mouse and rat. Validated for ELISA, ICC, IHC and WB.
Target
Mouse TXN / Thioredoxin / TRX
Synonyms
TXN | ADF | ATL-derived factor | SASP | Thioredoxin | TRX1 | Thioredoxin delta 3 | TRX | TXN delta 3 | TRDX
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived mouse Thioredoxin TRX recombinant protein (Position: V2-A105).
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • ICC (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TXN / Thioredoxin / TRX
P10599 NM_003329 NP_003320.2

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Featured Products

TXN / Thioredoxin / TRX Antibody - Anti-Thioredoxin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ TXN / Thioredoxin / TRX Antibody LS‑B7281
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Product
TXN / Thioredoxin / TRX Antibody LS‑C92747
Species:
Applications: Western blot, ELISA
TXN / Thioredoxin / TRX Antibody - Western blot of Thioredoxin, mitochondrial antibody at 2 ug/ml. Lane 1:293T whole cell lysate. Lane 2: EC109 whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 18 kDa. Observed band size: 18 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TXN / Thioredoxin / TRX Antibody (HRP) LS‑C212350
Species: Human
Applications: IHC, Western blot, ELISA
TXN / Thioredoxin / TRX Antibody - Western blot of recombinant TXN / Thioredoxin / TRX. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TXN / Thioredoxin / TRX Antibody (aa1‑105, Biotin) LS‑C297036
Species: Human
Applications: Western blot
TXN / Thioredoxin / TRX Antibody - Immunohistochemistry of paraffin-embedded mouse lung tissue.
TXN / Thioredoxin / TRX Antibody LS‑C409195
Species: Human, Mouse
Applications: IHC, Western blot, Immunoprecipitation
TXN / Thioredoxin / TRX Antibody - Western blot of Thioredoxin, mitochondrial antibody at 2 ug/ml. Lane 1:293T whole cell lysate. Lane 2: EC109 whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 18 kDa. Observed band size: 18 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TXN / Thioredoxin / TRX Antibody (Biotin) LS‑C212353
Species: Human
Applications: IHC, Western blot, ELISA
See all TXN / Thioredoxin / TRX Antibodies

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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/2/2024
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