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Catalog Number Size Price
LS-C160184-400 400 µl $393 
TRIM28 / KAP1 Antibody - TRIM28 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
TRIM28 / KAP1 Antibody - Fluorescent confocal image of HeLa cell stained with TRIM28 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TRIM28 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TRIM28 immunoreactivity is localized to Nucleus significantly.
TRIM28 / KAP1 Antibody - TRIM28 Antibody western blot of K562 cell line lysates (35 ug/lane). The TRIM28 antibody detected the TRIM28 protein (arrow).
TRIM28 / KAP1 Antibody - TRIM28 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
TRIM28 / KAP1 Antibody - Fluorescent confocal image of HeLa cell stained with TRIM28 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TRIM28 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TRIM28 immunoreactivity is localized to Nucleus significantly.
TRIM28 / KAP1 Antibody - TRIM28 Antibody western blot of K562 cell line lysates (35 ug/lane). The TRIM28 antibody detected the TRIM28 protein (arrow).
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Polyclonal Rabbit anti‑Human TRIM28 / KAP1 Antibody (aa178‑207, IHC, IF, WB) LS‑C160184

Polyclonal Rabbit anti‑Human TRIM28 / KAP1 Antibody (aa178‑207, IHC, IF, WB) LS‑C160184

Antibody:
TRIM28 / KAP1 Rabbit anti-Human Polyclonal (aa178-207) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C160184-400
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TRIM28 / KAP1 Rabbit anti-Human Polyclonal (aa178-207) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
KAP1 antibody LS-C160184 is an unconjugated rabbit polyclonal antibody to human KAP1 (TRIM28) (aa178-207). Validated for IF, IHC and WB.
Target
Human TRIM28 / KAP1
Synonyms
TRIM28 | E3 SUMO-protein ligase TRIM28 | KRIP-1 | Nuclear corepressor KAP-1 | KAP-1 | KAP1 | KRAB-interacting protein 1 | TIF1B | RNF96 | TIF1-beta | TIF1beta | Tripartite motif-containing 28 | KRAB-associated protein 1 | RING finger protein 96 | TF1B | Tif1beta zinc finger protein | Tripartite motif containing 28
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa178-207
Specificity
This TRIM28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human TRIM28.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TRIM28 / KAP1
Q13263 NM_005762 NP_005753.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/15/2024